Isolation and characterization of the Vibrio cholerae recA gene

The isolation and identification of Vibrio cholerae.

Isolation and characterization of the recA gene of Rhizobium meliloti.

Interspecific complementation of an Escherichia coli recA mutant with plasmids containing a gene bank of Rhizobium meliloti DNA was used to identify a clone which contains the recA gene of R. meliloti. The R. meliloti recA protein can function in recombination and in response to DNA damage when expressed in an E. coli recA host, and hybridization studies have shown that DNA sequence homology ex...

Nucleotide and deduced amino acid sequence of the recA gene of Vibrio cholerae.

The primer sequences reported in this paper were published incorrectly. The corrected sequences are listed below. Please note that the nucleotide sequence of one of the primers, LNS-CA2, was published incorrectly in this paper. The correct sequence is listed below. Please note that the EMBL accession number accompanying this paper is X61384 and not as previously reported.

Phylogeny of Vibrio cholerae based on recA sequence.

We sequenced a 705-bp fragment of the recA gene from 113 Vibrio cholerae strains and closely related species. One hundred eighty-seven nucleotides were phylogenetically informative, 55 were phylogenetically uninformative, and 463 were invariant. Not unexpectedly, Vibrio parahaemolyticus and Vibrio vulnificus strains formed out-groups; we also identified isolates which resembled V. cholerae bioc...

Characterization of Vibrio cholerae aerotaxis.

The ability to move toward favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular, aerotaxis has been extensively studied in Escherichia coli and was shown to be dependent on the aer and tsr genes. Three putative aer gene homologs were identified in the Vibrio cholerae genome, designated aer-1 (VC0512), aer-2 (VCA0658), and aer-3 (VCA0988). Deletio...